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No further improvement in the resolution of the averages upon further cycles, as assessed by the Fourier ring correlation or the spectral SNR criterion, is also an indication that the multi-reference alignment procedure is completed.
The alignment procedure is done iteratively over many cycles and is considered completed when overall image shifts and rotations no longer decrease upon further alignment cycles.
The whole decoding procedure is completed.
Complete design, calibration and alignment procedure is described.
The alignment procedure is described and results for the alignment precision and position resolution are reported.
In this study, the multiple structure alignment procedure is used.
The multiple alignment procedure is a modification of the pair-wise superimposition method outlined above.
The doctor squeezed my hand after the procedure was complete.
The averaging procedure and the complete alignment procedure are very robust: after the alignment the average trajectories were reproducing very closely the ground truth trajectory.
The final output of this alignment procedure was an offset that was then correlated to RT.
This final alignment procedure was iterated until optimal spatial matching was achieved.
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