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Sequence read alignment performed with an alternative aligner, Bowtie, yielded identical alignments for the large majority of reads and indistinguishable results in all downstream analyses.
Alignment performed with Clustal Omega and exported with Jalview software.
An alignment performed with Biology Workbench 3.2 at the San Diego Supercomputer Center SDSCC) [ 16] is shown in Fig. 1.
A multiple sequence alignment performed with the C-terminal regions (see Fig. 2) allowed the establishment of phylogenetic relationships among all proteins (see Material and Methods).
A protein sequence alignment performed with the ClustalW algorithm was used to construct a phylogenetic tree using the neighbor-joining method in the MEGA package [ 56].
Data from positive and negative ionization modes were separately processed as follows: minor differences in retention times were corrected by peak alignment performed with XCMS version 1.5.2 [ 38] run under R package 2.4.0.
Similar(51)
Percentages of nucleotide identity were calculated from alignments performed with MUMer v3.0 [ 67] and genome maps were constructed using in-house scripts.
Retention time alignment was performed with a time alignment window of 20 min and a retention time match window of 0.5 min.
Alignment was performed with BLASR28 -bestn 3, and primary aligned reads with mapping quality of 254 were used.
Alignment of sequence assemblies with the BNG map was computed with RefAligner, and visualization of alignment was performed with snapshot in IrysView.
The alignment was performed with software BLAST.
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