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The form of perimysial collagen organization at different transmural locations was quantified on cross-fiber planes in all seven hearts using the measure of local collagen alignment outlined in methods.
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The procedure for extracting evolutionary information from sequence alignments is outlined in Figure 1.
In addition, one may want to use automatically produced local alignments as anchor points to speed up the alignment procedure as outlined in [ 18].
Our alignment strategy is outlined in Figure 22.
While efficient, the alignment-recycling method, AR-0.73, outlined in this work does have its limitations.
Strand invasion artifacts were removed post-alignment using the same procedure outlined in [ 9] with some minor modifications.
A sRNA-encoding gene was considered to be present or conserved in a given genome if BLAST searches yielded one or more alignments satisfying the similarity criteria outlined in Methods.
Future efforts will aim to develop programs that speed up the construction of haplowebs from FASTA sequence alignments and help perform the congruence analysis outlined in this article.
For this purpose, we developed a PCAIN-based scoring scheme (Figure S6) outlined in the methods section for template selection, anchored sequence alignment, and homology-based structure prediction.
The alignments were run according to the barcode plate strategy, as outlined in the GS-FLX Shotgun DNA Library Preparation Method Manual (supplied by Roche Diagnostics, Roche Applied Science, Mannheim, Germany).
Finally, the alignments were searched for SNPs using PolyPhred [ 15] as outlined in Figure 1B.
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