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2. Sequence alignment on Figure 1 would benefit from inclusion of sequences of several ATP-grasp superfamily members of known 3D structure, such as RimK (4iwx), LysX (1uc9), glutathione synthase (1gsh), and/or biotin carboxylase (1dv1).
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Starting with many more sequences, we would have faced the following problems: 1) The alignment showed on Figure 2, which was used to generate the trees, could not have been provided within the manuscript.
The most conserved parts of the alignment are shown on Figure 2. The fragment of Leifsonia xyli CTCB07 genome [GenBank: NC_006087] revealed by Genomic BLAST has 2 stop codons in the region homologous to genes of GH97 family proteins.
(A ) The CENP-M alignment identifies highly conserved residues (based on alignment in Figure 3 figure supplement 1, panel D ), a subset of which (asterisks) is exposed at the surface of CENP-M.
This is shown by the complete alignment on the dot plot (Figure 2B) where the 5 large assemblies of the Brachypodium v1.0 sequence (Chromosomes 1 5) aligned with the five assemblies of FPC contigs (A.A.1-E.E.1).
Note: Position of the SNPs are based on sequence alignment (supplementary Figure 1).
Pairwise amino acid sequence identities of KatG sequences from different Symbiodinium ITS2 types based on the alignment in Figure 5.
Pairwise amino acid sequence identities of APX sequences from different Symbiodinium ITS2 types based on the alignment in Figure 3.
Pairwise amino acid sequence identities of MnSOD (#1-21) and FeSOD (#22-25) sequences from different Symbiodinium ITS2 types based on the alignment in Figure 1.
To highlight this, we also marked the residues in the "a" and "d" positions of the coiled-coil heptad repeats on the alignment in Figure 1 figure supplement 1A, as requested.
Figure 16 Results of image alignment on real images.
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