Sentence examples for alignment of unique from inspiring English sources
(XLS 33 kb) Additional file 2: Amino acid alignment of unique MHC class I sequences from each population.
A spectrogram alignment of unique units revealed striking regions of nucleotide preference in several places within the variable regions (Fig 2).
The first SNP prediction method, for testing and validation involved the use of GMAP software [ 13] for alignment of unique PI 468916 short sequence reads to the Williams 82, preliminary 4× scaffold assembly, with SNPs predicted from the alignments.
In short sequences were trimmed to include a uniform length of 350 assembled at 99% similarity using NGEN (DNAstar, Madison WI), alignment of unique sequences performed with Muscle [ 58], distance matrix created using DNAdist algorithm of the Phylip package [ 59], and PCA analysis performed in R (R Development Core Team).
Multiple alignments of unique sequences from each sample were carried out using T-Coffee [ 34- 36].
After identification and elimination of artefactual sequences, we determined 18, 3, 4 and 5 putative alleles for each species (alignments of unique sequences and putative alleles are included as the Additional file 1 and Additional file 2).
Alignments of unique sequences and alleles are shown in Additional file 1 and Additional file 2. Out of the 24 loci expressed by these four Conus species, 22 appear to represent functional genes because predicted amino acid sequences represent potentially active α-conotoxins based on the presence of an intact cysteine framework.
The alignment of each unique Ig-like domain from LigA (six common with LigB and seven LigA-only), LigB (six unique), and LigC (six unique) suggests that the secondary structure of LigB-12 is shared by the family of 31 domains.
For instance, on the basis of an alignment of 344 unique sequences, Fu and Chang identified a highly conserved P-G doublet in non-animal sHSPs [36].
These were excluded resulting in a final alignment of 56 unique primate mtDNA genome haplotypes.
Additional file 4: Figure S13 Alignment of all unique sequences from AhLOX7 3' amplification (2186/2187) demonstrates sequence variation.
