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Two different scores estimate the accuracy of an alignment comparing to the BAliBASE reference alignment: the Sum-of-Pairs score (SP), and the Total-Column score (TC).
Total DAPs consisted of the molar sum of total DMs and total DEs.
To calculate the relative error rates in the PacBio data from the BLAT alignment of the PacBio data against the chloroplast consensus sequence, the number of mismatches in the alignment was summed and divided by the total number of nucleotides in the alignment using a custom Python script (Additional file 2).
It was an alignment of the stars.
Accuracy of alignment was calculated with the two standard scoring functions provided by BAliBASE, the sum-of-pairs and total-column scores, and computational costs were determined by collecting peak memory usage and time of execution.
The benefit of a higher total exposure is not apparent in the alignment of rotavirus DLP because the alignment errors of all tested frame sums is extremely small.
DOI: http://dx.doi.org/10.7554/eLife.06980.009 The higher SNR present in exposure-filtered frame sums, compared with unfiltered sums containing a lower total exposure, is expected to help in the alignment of smaller particles where the alignment accuracy is significantly limited by the signal generated by the particle.
The score of the final alignment is then summed over all column scores.
Calculation of conservation of CICPs within the alignments is calculated per alignment position by summing up the CICP occurrences per column and dividing by the total number of sequences that participated in the CICP study for that alignment.
Second, sum of the integrated results yields the total power.
One is the alignment of planets.
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