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Finally, although extreme care has been taken during the alignment of the sections, small differences cannot be ruled out since there are inherent differences depending on the way in which the sections were mounted on gelatin-coated slides by our technical personnel.
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In order to verify the alignment of the section across subjects, the contour of the entrance to the LV was digitally reconstructed and the maximum feret length was statistically compared (ANOVA) between conditions.
3D reconstructions of the histological volumes were computed using the following three datasets: (i) the previously ascertained 3D-MRI scan, (ii) images of the paraffin block face obtained during sectioning for the precise alignment of the histological sections and (iii) the digitized images of the cell body-stained sections (Amunts et al. 2004).
This is caused by unavoidable eccentricity of the setup such as irregularities in the surfaces of contact, non-alignment of the section and of the jack.
The relative error with respect to analytical results for capillary cross-sections of circular, triangular and square shapes are calculated as a function of the cross-section diameter, a, and for different alignment of the cross-section relative to the underlying lattice grid.
These variations together with the depth and alignment of the cross section along the strait dominate the spatial and temporal variations and sometimes sharp changes in the flow pattern in three dimensions.
This could potentially cause bias in the alignment of the cross-sections, especially ones with considerable noise, and it would require much greater computational time to determine a window for each cross-section of each projection.
Due to the concentration difference, photo-carriers diffuse into the Si NWs, followed by being extracted to the electrodes by the built-in electric field in the NW, together with the photo-carriers generated in the NWs. Figure 3b presents the equilibrium band alignment of the cross section of the hybrid structure.
Any read with an alignment of the nonretrotransposon section plus 10nt was removed as a potential molecular chimera.
A subsequent alignment of the PEPCK intron section was done using MAFFT v. 5 [52] in Jalview.
We took advantage of this feature to create "virtual" fiducial markings through the anterior and posterior commissures (as well as the corresponding orthogonal axes) that were used for the alignment of adjacent sections.
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