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For visualization of blastx results, we imported the top tomato P450 hit of each of our transcripts, along with the bit score of the blast alignment to the program Cytoscape (P. Shannon 2003).
Specific nucleotide variation between and among protein-encoding genes and within non-coding regions was assessed by comparative alignment of each genome using the Smith-Waterman alignment algorithm of the program Burrows-Wheeler Aligner (BWA: [ 46]).
At this reference set, the alignment of sequences with linear motifs generated by MUSCLE might be facilitated by Kimura's distance, the second stage in the progressive alignment of this program.
To evaluate the expression of unigenes, we first mapped trimmed reads to unigene sequence using gapped alignment mode of the program Bowtie 2.2.1.0 (Langmead et al. 2009).
For the alignment of the proteolytic fragments, the program FindPept (http://www.expasy.ch/tools/findpept.html) was used.
To consider pairs of alignments together, the program imposes successively stronger constraint levels and attempts to align each end at the given constraint level.
And while formal women's networks do exist -- even commonly -- the alignment of formal programs with the networks that are truly pushing women forward in their careers is often lacking.
Visual inspection of the alignments using the program MEGA1.4 [ 63] helped in refinement of the final alignments.
For the generation of multiple sequence alignments (MSAs) the program M-Coffee [ 32] was used.
For coding regions, we first translated each gene into amino acid residues, which are less divergent as changes occur at a lower rate of substitution, to serve as a "scaffold" for alignment of nucleotides using the program RevTrans [ 29].
The comparisons were computed as local alignments using the default parameters of the program [ 21].
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