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The phrase "alignment of the largest" is grammatically correct and can be used in written English.
It can be used in contexts where you are discussing the arrangement or positioning of the largest items, entities, or elements in a particular setting or system.
Example: "The alignment of the largest structures in the city was crucial for the urban planning project."
Alternatives: "arrangement of the largest" or "positioning of the largest".
Exact(2)
(b) and (c) alignment of the largest OCDC contours.
Mauve produced LCBs with the longest average length (Table 3, Supplementary Fig. S1) but did not complete the alignment of the largest data sets used in this evaluation in the allotted time.
Similar(58)
This effect was attributed to the optimized alignment of the large 2D GnP flakes within the amorphous soft polymer.
Bayesian analysis using the 5.8S re-included ITS alignment of the large data set without manual adjustment produced a phylogeny with moderate to strong support for many internodes (Fig. 5).
Alignment of the large chromosome of Nmn.
Compared to the small data set, the alignment of the large data set contains more taxa, more genes, but a smaller fraction of genes present per taxon.
Li et al. pointed out that the better alignment of emitters, the larger the emitting current density [33].
Primers LR1 and NDL22 were designed from previously published alignments of the large ribosomal subunit [66] flanking the variable domains D1 and D2.
Exon conservation was determined by the score (Sort) from CLUSTALW alignments, self-alignment of the larger exons to yield the score Sid, and calculation of the normalized score Stot = Sort/Sid.
In fact, despite length differences between sequences of Attagis, Thinocorus, and Pedionomus, the alignment of these large insertions could be taken as further evidence of the close relationship of these two families.
The obtained global alignments, combined, will give the best local alignment of the two large sequences.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com