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An alignment of subject pairs 12 and 16 is presented in Figure 2B as a simplified charge map (with basic charges shown in blue).
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Alignment of the subject was achieved with respect to the anterior corneal specular reflection, while the subject fixated on a reference E-letter target projected on a minidisplay at optical infinity.
Finally, we present anecdotal results on the alignment of two subject MRI brain data.
This creates a dark fixation dot against an illuminated background which was used for alignment of the subject.
These effects can be dependent on alignment of the subject relative to the OCT device, so different alignment might cause opacities to shadow different parts of the retina in subsequently acquired volumes.
Finally, yet importantly, volumes that cover a larger field of view can be especially affected by vignetting that is again dependent on alignment of the subject's iris with respect to the OCT beam.
TBSS was used for voxelwise analysis because TBSS may overcome potential problems with the registration and alignment of white matter between subjects in order to allow cross-subject statistical analysis [31].
In the latter case, the tentative barley transcript was manually assembled from the output of the BLASTn alignment of the barley subject sequence detected by the wheat Ta- Clo11 caleosin query sequence.
To minimize any influences of subject alignment on the measurement, 10 3datata sets were recorded at exactly the same location on the retina (~4° eccentricity nasal from the fovea) of healthy volunteers for every time point in the time series.
There was good alignment of non-fatigued subjects with 90% of empiric NF controls residing in LCA classes 0 (Well) and 2 (Unassigned).
However, intuitively the alignment of each selected control subject to its matched case could be inefficient, since that subject may also properly serve as a member of the comparison group for cases occurring at a range of other times.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com