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Strain-dependent calculations based on k·p and configuration interaction models show that the minimum reachable FSS highly depends on the alignment of stress with respect to the QD elongation direction (compare also with Figure 3b,c).
Furthermore, the mechanisms by which tension contributes to the alignment of stress fibers and actin dynamics within these actomyosin bundles have not been reported.
Future studies could now examine whether the newly identified pathway, which allows mechanical forces to control the assembly and alignment of stress fibers, is conserved in other cell-types.
Finally, it will be interesting to examine how mechanosensitive actin filament assembly and disassembly contribute to generation and proper alignment of stress fibers and other contractile actomyosin bundles in the tissue environment.
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It is, however, important to note that the stress fiber network is typically poorly organized in VASP-depleted U2OS cells, suggesting that dorsal stress fibers are required for proper alignment of ventral stress fibers in migrating cells.
Collectively, our findings reveal that mechanosensitive actin filament assembly and disassembly are essential for generation of contractile ventral stress fibers, and function as selection processes to ensure proper alignment of ventral stress fibers perpendicularly to the direction of cell migration.
These data provide support to a new mechanobiological model explaining the principles of assembly and alignment of ventral stress fibers in migrating cells.
Taken together, these data reveal that myosin-derived tension precisely controls both actin filament assembly and disassembly to ensure generation and proper alignment of contractile stress fibers in migrating cells.
A multiple alignment of some stress-NAC TFs [ 15] by DNAMAN revealed that CiNAC3 and CiNAC4 shared a highly conserved N terminal DNA binding domain, named NAC domain which consists of 5 consensus sub-domains, and a highly variable C-terminal transcriptional regulation domain (Fig. 1).
Application of biomechanical stimuli results in remodeling of the cell body mainly through alignment and generation of stress fibers (Wang et al. 2001).
(4) AMPK-mediated VASP phosphorylation is necessary for assembly and proper alignment of contractile ventral stress fibers.
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