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To overcome a bias in alignment of short reads, where the reference allele reads are preferentially aligned over reads with the variant allele, we used a genomic reference containing IUPAC codes for SNPs in dbSNP.
Schneeberger, K. et al. Simultaneous alignment of short reads against multiple genomes.
Prüfer, K. et al. PatMaN: rapid alignment of short sequences to large databases.
Langmead, B. et al. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M. & Salzberg, S.L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
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However, despite their advantages, several technical issues – primarily sequencing errors, insufficient coverage of sequences, and potentially anomalous alignment of short-read sequences, have been known to confound the identification of authentic editing sites [ 21, 22].
We first explain our methods for (i) listing possible extensions for a given e-node in the e-graph, (ii) scoring each extension based on the alignment of short-insert read-pairs and (iii) scoring each extension based on the alignment of long-insert read-pairs.
They do not report, however, using the MeSH itself as a parallel resource for alignment of shorter terms.
To determine whether the approach also worked for the alignment of shorter reads, the first 240 bases of each BAC end sequence were also aligned to the equine genome.
This may be due both to the presence of multiple alternative contigs produced by Velvet/Oases for many transcripts and to the alignment of shorter contigs to different parts of the same protein.
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