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Orthologous sequences were manually aligned following the procedure used earlier in the alignment of over 700 globins [ 74].
Alignment of over 30 enterobacterial prfC-like genes indicates that the primers designed to amplify attR junction would amplify IncJ element: host junctions from a wide variety of hosts.
Although it is nearly impossible to manually adjust an alignment of over hundreds divergent ITS sequences, there are segments of appreciably misaligned data in highly conserved regions in even the best-scored SATé alignment.
The extremely high AT content of Dictyostelium introns and the help of a multiple sequence alignment of over 1700 myosin motor domains (M. Kollmar, unpublished data) nevertheless allowed the unambiguous identification of the introns and, subsequently, the protein coding regions.
The alignment of over 70,000 exons common to mouse and rat predicts a synonymous substitution rate of 0.17 [ 16] over 10-fold greater than the Rhox2 paralogues with a dS value of 0.0122 and 100-fold greater for the Rhox4 paralogues at 0.001.
Three such co-evolutionary analyses were performed (using a web-based interface) [ 22] on a multiple sequence alignment of over 100 ABCG family sequences, namely statistical coupling analysis [ 25], Chi-squared test [ 27] and explicit likelihood of subset co-variation [ 28].
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Groups of homologs were built using the criteria that sequences were homologous if the Best Blast Hit (BBH) showed an alignment length of over 70 amino acids and with a percent identity over 20%.
Alignment of the over five millions of 25-bp short reads to the bovine genome identified over 62,000 putative SNPs with a 91% validation rate.
A single wrong fragment accepted at an early stage of the greedy procedure can subsequently prevent many correct fragments from being included into the alignment, thereby leading to an output alignment of lower over-all quality and to instability.
JA performed the 16 S rRNA alignment, analysis of over-represented COGs and recombination.
Alignments of over 100 sequences (from NCBI database) of each gene region were carried out, respectively.
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CEO of Professional Science Editing for Scientists @ prosciediting.com