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To further verify structural distribution of nsSNPs in functional kinase regions, we also performed structure-informed multiple alignment of kinase sequences using PROMALS3D approach [129].
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Proteins were defined as "kinase" based on significant match to a Hidden Markov Model (HMM) generated from an alignment of eukaryotic kinases (kinase.com).com
Motif-based alignments of kinase sequences to the catalytic core were first generated by implementation of the Gibbs motif sampling method [125], [126].
Analysis of the rice MAPKKKs along with the Arabidopsis for Raf specific signature GTXX (W/Y) MAPE was carried out by multiple alignments of kinase domains.
Multiple sequence alignment of the kinase catalytic domains of the putative human protein kinases has been carried out using ClustalW [ 42].
The obtained alignment was then matched against the alignment of the kinase sequences with the available crystal structure to ascertain the quality of the sequence alignment.
In one [29], Gloor et al. used local covariation (as in Figure 2) to improve a structure-based sequence alignment of phosphoglycerate kinase.
To find out the relation between the different DAPK family members we calculated a phylogenetic dendogram from an alignment of the kinase catalytic domains from DAPk, DRP-1 and ZIPk.
A full alignment of the kinase domains is shown in Additional file 1.
Multiple sequence alignment of the kinase domains (generated using clustalW) has been used as input.
Additional File 6 contains a multiple sequence alignment of the kinase domains of all 76 identified subfamily members.
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