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Bayesian majority-rule mid-point rooted tree of an amino acid alignment of expressed transcripts in Maxillipoda.
Given intronic locations deduced from alignment of expressed sequence tags (ESTs) to the genome, ASPic-GeneID attempts to predict complete gene structures in the target genome sequence.
However combining the networks generated by sequence alignment of expressed miRNAs and Targetscan, we predict that HNF4A is indirectly regulated by the let-7 family of miRNAs.
Brid., we have generated primers for exon-primed intron-spanning loci, based on an alignment of expressed sequence tags (ESTs) from C. purpureus to the Physcomitrella patens (Hedw).
Current methods for predicting genes using ESTs generally work by first performing an alignment of expressed sequence tags onto a target genomic region and then combining the alignment results with ab initio gene predictions [ 22].
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PASA is a genome annotation tool that exploits spliced alignments of expressed transcripts to model gene structures [27].
These probes were originally selected from the consensus sequence alignments of expressed sequence tag (EST) sequences.
An alignment of 67 expressed plant serpin sequences showed that the motif Y XXG XD XR XF was present in 54 of these sequences, with an additional eight sequences containing conservative variations of the motif [ 7].
Putative exons and open reading frames (ORFs) were predicted by the programs GenMark.hmm [ 54], FGeneSH [ 55] and by alignment of EST (Expressed Sequence Tags) and protein sequences using GenomeThreader [ 56].
Based on the alignment results, we identified the set of expressed transcripts and estimated their expression levels.
In this study, alignment of current EST and genomic sequences revealed that ~10% of expressed genes are alternatively spliced in Mt compared with 20% in At and Os.
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