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Alignment of combined multilocus sequences covered 15,717 bp and 968 polymorphic sites, including 362 segregating sites and 606 indels.
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The final alignment of the combined data set consisted of 4366 characters (positions).
The alignment of the combined nrLSU, rpb2 and ef1-α dataset, which was compiled only for the molecular dating analysis, consisted of 57 sequences 2428 bp in length.
Alignment of the combined 196,083,881 pre-processed sequencing reads generated a dataset of 163,078,230 mapped reads (83.2%), ranging from 5 to 48 nt.
Alignment of the combined chromosome and pPANA1 nucleotide sequences also showed that extensive synteny exists among the four complete P. ananatis genomes (Additional file 3: Figure S1).
Alignment of the combined dataset from PreSET and SET domains resulted in a matrix with length of 228 sites after removing ambiguous regions and autapomorphic insertions (see Additional file 3).
According to different gap selection criteria in Gblocks, the alignment of the combined dataset comprised 15688 (allowed gap positions = all), 15038 (allowed gap positions = with half) and 12570 (allowed gap positions = none) positions.
Circular maximum likelihood phylogenetic tree of combined alignment generated from UL27 sequences produced in this study and available sequences found at GenBank database.
Four types of combined alignments were generated, adding different sets of N-1 sequences to the native alignment.
Combined horizontal and vertical alignments are frequently used in mountainous freeways in China; however, design guidelines that consider the safety impact of combined alignments are not currently available.
This concept presented analysis of combined component alignment and its effect on patellar kinematics intraoperatively using patellar navigation could help to prevent maltracking of the patella and therefore postoperative complications.
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