Exact(3)
Finally we concatenated all the cleaned alignment files, obtaining a multiple alignment of about 5000 7000 columns.
Full-length 16S rRNA gene sequences (> 1300 bases) were added to an ARB alignment of about 28,000 small-subunit rRNA sequences by using the alignment tool ARB_EDIT of the ARB program package [ 37].
These genome-scale comparisons are becoming routine and the approaches can be divided into two main categories, alignment-based (for example, an alignment of about 400 universal proteins identified by Segata et al. 2013) and alignment-free (for example, the "google DNA" method described recently; Gautier and Lund 2013).
Similar(5)
It is interesting to note that clade 2-cluster 4 contains only 8 Arabidopsis proteins with an alignment identity of about 70%, of which six are on chromosome 3, while clade2-cluster 3 contains 6 Solanum ABCA proteins with an alignment identity of 90%.
To study the phylogenetic relationship of the 45 HvWRKY proteins we performed a multiple sequence alignment of the about 60 amino acids spanning the WRKY domain of all HvWRKYs.
The significant improvement in both out-of-plane and in-plane alignments of about 4° and 1.5° was observed compared with the substrate.
A genome-wide homology search was performed using the discontiguous megaBLAST algorithm http://blast.ncbi.nlm.nih.gov/Blast.cgi with a word size of 11 and rewards and penalties (2, -3) that optimize for alignments of about 85% identity.
The Clustal W alignment of the N-terminal ends of about 60 amino acids corresponded to each of the alignments obtained by the PSI-BLAST searches between the query and hit sequences (Fig. 3A).
Related(1)
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