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Multiple sequence alignment of ZmLTPs.
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The amino acid sequence alignment of the 8CMs of ZmLTPs reveals a variable number of inter-cysteine amino acid residues (Table 1).> -wrap-foot> Character "X" represents any amino acid, and the Arabic numeral following "X" stands for the numbers of amino acid esidues.
To clearly understand the sequence characteristics of ZmLTPs, we further analyzed the pI (isoelectric point) values, Mw values, and CXC motifs of all available ZmLTPs.
The analyses of putative upstream regulatory elements showed both shared and distinct transcriptional regulation motifs of ZmLTPs, further indicating that ZmLTPs may play roles in diverse biological processes.
Nearly 80% of ZmLTPs contained Skn-1_motifs which might account for endosperm expression.
Nearly 60.32% (38 of 63) of ZmLTPs were detected by RNA-seq.
The analyses of putative upstream regulatory elements further indicated that ZmLTPs may be involved in diverse biological processes and provided new insights into the shared and different transcriptional regulation machineries of ZmLTPs.
These conserved hydrophobic or hydrophilic residues may play significant roles in the biological functions of ZmLTPs [ 29].
To further dissect the biological function of ZmLTPs in cold tolerance, we investigate the possible roles of ZmLTPs under cold stress condition in two inbred maize lines, chilling-sensitive ETH-DL3 and chilling-tolerant ETH-DH7 [ 49].
Chromosome 1 contained the maximum number of ZmLTPs (11), while the minimum number (2) were presented on Chromosome 9.
Nearly 66.67% (42) of ZmLTPs were reliably detected by RNA-seq data (Additional file 18: Table S13).
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