Exact(9)
These alignment metrics serve as a measure of transcriptome accuracy.
Broad alignment metrics are shown in Table 1.
Sequencing and alignment metrics for each young and old liver sample.
Despite the limited sequence data available for T. bernacchii, these alignment metrics indicate a relatively accurate transcriptome assembly.
Finally, the algorithms for absolute optimum, with respect to selected alignment metrics, have also been published [ 1, 23], but they are computationally too expensive for everyday use.
There is a positive correlation between high value sequence assembly metrics and in silico map-to-genome map alignment metrics (Additional file 6: Sections 3 5) as well as post super scaffolding N50 improvement (Additional file 6: Sections 3,5).
Similar(50)
The Picard command CollectMultipleMetrics was employed to each of the merged bam files to provide information on alignment summary metrics, insert size metrics and quality by cycle metrics.
In a routine quality control (QC) procedure on verifying the alignment, QC metrics including insert-sizes, mapped reads, unmapped reads and reads that align with a deviated pattern (DP; discordant read alignments) were examined.
Sequence and alignment quality metrics for each patient and PE pulse are available in Additional file 1: Table S2-S3.
The consensus from the sequence reads will then be taken as the new sample's genome and SNPs are called using sophisticated statistical methods based on sequence and alignment quality metrics.
We apply our approach to detect conserved protein complexes in yeast fly and yeast worm PPI networks, and show that our approach outperforms two recent approaches in most alignment performance metrics.
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