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The alignment method of the other two phylogenetic analyses was implemented on MUSCLE 3.6 [ 75] and the conserved region of each alignment was trimmed using Gblocks [ 76], which allows less strict flanking positions and gap positions within the final blocks, but does not allow many contiguous nonconserved positions.
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Third, the alignment method of this approach is very simple.
The BLAT alignment method of identifying gene homologues in the four strains limits our analysis to less diverged genes.
NATALIE, the alignment method of NATALIEQ, is applicable to any type of network and supports any additive score function taking both node-to-node correspondences and topology into account.
We chose to employ the multiple alignment method of [ 25] who applies partial order graphs (POG) to the multiple alignment problem.
The alignments were manually modified to correct defects from the automatic alignment method of ClustalW.
Sequence alignments were generated with the optimal alignment method of Vector NTI software.
In future, instead of using TopHat2 for junction alignment, we will consider assessing the influence of different junction alignment methods on the performance of IRcall and IRclassifier.
Figure 2 shows results comparing DACTAL to the default 24-h analysis using SATé and to two-phase methods (RAxML analyses of leading alignment methods) on the more difficult 1000-taxon model conditions.
In order to increase the computational efficiency of the proposed network alignment method, instead of using the original transition probability matrix P, we use a reduced matrix P ˜.
We used the multiple sequence alignment methods (CLUSTALW) of the DNASTAR suite program MegAlign (Lasergene, v7.2; DNASTAR, Madison, Wisconsin, USA) (data not shown).
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