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The alignments were manually modified to correct defects from the automatic alignment method of ClustalW.
When evaluated using the challenging Maximum Unbiased Validation dataset, shape constraints were able to extract significantly enriched subsets of compounds for the majority of targets, and FOMS matched or exceeded the performance of both VAMS and an optimizing alignment method of shape similarity search.
Third, the alignment method of this approach is very simple.
Sequence alignments were generated with the optimal alignment method of Vector NTI software.
The BLAT alignment method of identifying gene homologues in the four strains limits our analysis to less diverged genes.
We chose to employ the multiple alignment method of [ 25] who applies partial order graphs (POG) to the multiple alignment problem.
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We compare the accuracy of alignment methods existing at the time of this work and the new alignment method on datasets simulated to encompass a broad range of genomic mutation types and rates, including inversion, gene gain, loss, and duplication.
Alignment methods of nematic liquid crystals (LCs) by surface photoreactions on substrate surfaces were initially proposed around 1990, and the photoalignment technology of nematic LCs has recently been integrated into the LC device fabrication industry due to its profitable features.
In future, instead of using TopHat2 for junction alignment, we will consider assessing the influence of different junction alignment methods on the performance of IRcall and IRclassifier.
Next section will detail the properties of the proposed alignment method for this kind of data.
We then apply the multiple genome alignment method to a group of 23 finished genomes in the family Enterobacteriacae (Table S1).
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