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This tool assembled the BAM (binary alignment map) and BAI (binary alignment index) files and aligned them to the rCRS using TMAP Smith Waterman alignment algorithm [22].
Data were aligned to the hs37d5 genome [ 23], producing compressed Binary Alignment Map format files.
Alignments were converted from the sequence alignment map (SAM) to binary alignment map (BAM) format.
It takes alignments to the genome as input in sequence alignment map (SAM) or binary alignment map (BAM) format [ 10].
Alignments were converted to sequence alignment map format followed by binary alignment map, sorted and indexed (Samtools version 0.1.19).
The resulting sequence alignment map (SAM) files were converted to binary alignment map (BAM) files, and subsequently sorted via SAMtools.
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BM-Map takes SAM format (Sequence Alignment/Map) [ 16], the most popular read alignment format, as the standard input.
SAM alignment files were further processed to create pileup files using the SAMtools package (Sequence Alignment/Map format tools, [40], http://samtools.sourceforge.net/).net/
The software package SAMtools was used to convert the sequence alignment files from sequence alignment/map (SAM) to sorted binary alignment/map (BAM).
In addition, BWA outputs alignment in the new standard SAM (Sequence Alignment/Map) format.
The default output alignment format is SAM (Sequence Alignment/Map format).
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