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In this study, we have performed RIPCAL analyses using 3 different models: highest G C content, alignment majority consensus and predicted sequence of the repeat family prior to RIP-mutation.
RIP dominance [ 20] was calculated using a variety of comparative models including: sequence of highest G C content; alignment majority consensus and; consensus sequence predicted by deRIP (Additional file 5).
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In the first group (upper 5 lines in the alignment), the majority of cysteines are arranged in clusters of two or three consecutive residues.
The assignment of assembled sequences to chromosomes was done based on a best alignment and majority rule.
We then called a site characteristic if in the combined S1-S2 alignmenthehe majority of sequences from one group share an amino acid or an insertion/deletion that is not seen in the respective other group.
produced much improved alignment of the majority of statistics to their expectations (Figure 3b).
The datasets aligned to the correct reference at a rate of 0.51 to 2.04 using the LAST alignment, but a majority of the top identifications were correct (Table 2).
Alignments containing a majority of gaps and missing data were not considered for subsequent analyses.
In contrast to the majority of alignment tools, DIALIGN constructs a multiple sequence alignment (including a quality score for each alignment column) from whole sequence fragments found in local pair-wise alignments.
Few clades were supported by the majority of alignment blocks, indicating that combining information from multiple alignment blocks for inference in BUCKy does little to increase support for clades, especially those that are basal.
Approximately 90% (depending on the applied FDR) of genes under positive selection based on the PRANK alignments are also detected when using MUSCLE alignments, suggesting that the majority of positively selected genes are consistent between the two alignment approaches.
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