The phrase "alignment limits" is correct and usable in written English.
It can be used in contexts related to engineering, design, or any field where parameters or boundaries for alignment are discussed.
Example: "The alignment limits must be adhered to in order to ensure the proper functioning of the machinery."
Alternatives: "alignment boundaries" or "alignment constraints".
Although new high-throughput sequencing techniques are a promising approach to absolve the need for "strain-" and "species-specific" microarrays, difficulties in transcriptome assembly and the lack of reference genomes, required for reads alignment, limits comparative studies on transcriptome structure.
First, they have lower than average information content per position (i.e. less conservation), and require longer alignments in order to produce a significant hit, but the requirement of ungapped, unbranched alignment limits the possible length of hits.
For in-house genomes, SNP calls were then made by using SolSNP version 1.1 with the following alignment limits: minimum coverage of 20, minimum mapping qualities of 20, and a filter call of 0.95.
However, we observed occasional differences among ancestry groups that will merit attention to separate technical noise (sequencing, alignment, limited data for some populations) from biologically relevant differences.
The above inferences were based on the alignment limited to the previous reconstructions [ 12], which is only supported by tRNAscan-SE for D. pteronyssinus (Additional file 5, 19, 20).
Likewise, no stable trees could be obtained from the subsection of the alignment limited to the conserved motifs, e.g. absolutely reliable regions, perhaps due to the insufficient number of compared positions (data not shown).
Identification of exons via tiling of read alignments limits the size of the exons found to the length of a single read.
Of note, heuristics applied in short read aligners limit alignment of pairs within a given distance range.
This suggests that post-mortem deaminations at cytosine residues contribute massively to the edit distance of alignments, limiting our ability to identify the fraction of endogenous reads that are most divergent.
As seen in (Additional file 6: Table S5), for 39 domains with potential enzymatic role, short length of the query domain and alignments limited the scope of function recognition.
First in Stuttgart, Germany, then for two decades as director of Frankfurt Ballet, he took the genre in new directions by both ignoring and exploring its conventions — taking positions out of classical alignment; testing limits of balance, extension and strength; inventing strategies to generate movement; adding text, film and innovative lighting.
