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Second, the genome alignments are targeting genome scale sequence similarity and thus may sacrifice the alignment quality of short functional regions.

There are other software packages that offer some overlapping functionality with BoulderALE, but they are targeted for different alignment problems.

RaxML tree and alignment analyses confirm that cytoplasmic actin was targeted in our RNAi knockdown experiments and thus we expect cytoplasmic actin specific phenotypic effects.

Although spliced alignment is less sensitive than targeted alignment, it does have the ability to find novel splicing at locations not included in a database of known splice sites.

But for intergenic splicing events in general, targeted alignment is not applicable as a computational strategy, because it is infeasible to generate all possible exon-exon pairs over the human genome.

Additionally, one of the species in the tree (but absent in the multiple alignment) is chosen as the target species for the reconstruction.

Target sequences are threaded through the PDB by eight LOMETS programs, and a multiple sequence alignment is constructed based on the target sequence (with external inserts/gaps shaved).

The best scored target-probe alignment is tested to predict a high hybridization signal.

A multiple sequence alignment is first performed using the target sequence and a large number of homologous sequences.

Hence, to facilitate the identification of regions of the model expected to be of high quality (i.e. highly conserved sequence alignment with no or few insertions and deletions), the structurally derived target-template alignment is shown in colors, highlighting regions of conservation between the modeled protein and the structure template (41).

Optimal template selection, and target-to-template alignment is key to the success of any modeling exercise [40], [41].

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