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The protein space can be visualized as a graph-like structure, where nodes represent proteins and a significant alignment is saved as an edge between two nodes.
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When a conformer is overlaid on a reference shape, and the computed shape Tanimoto is above the Transform-Tanimoto, the data required to reproduce that alignment are saved (Figure 4).
The alignment was saved as a Phylip file and the graphic was generated using Version 6.2 of CLC Sequence Viewer (www.clcbio.com).
The alignment was saved in NEXUS format for the phylogenetic analysis.
Where multiple targets within a database produced alignments with significant E-values, the single best-scoring alignment was saved.
The alignment was saved as a PAUP file and used to generate a phylogenetic tree using PAUP 4.0b6.
The duplicated segments identified for each pairwise alignment are saved as an output file in MAF format.
For each minicircle sequence, optimal local alignments were performed against the fully edited sequence (predicted or actual) of every edited maxicircle gene and the information from the best alignment was saved to determine its significance (Fig 3A).
The resulting multiple alignments are saved in MAF format for each LCB.
Locus-Assigner then generates pairwise sequence alignments for each cDNA sequence/reference sequence pair using CLUSTAL W. Pairwise alignments are saved as separate files.
The alignments were saved and executed by MEGA Version 5.0 to generate a Neighbour Joining Tree with bootstrapping (5000 replicates) analysis and handling gaps with pairwise deletion.
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