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For all "SM" probe sets, the number of probes perfectly matching the genomic sequence within the region defined by the probe set consensus alignment is reported.
Percent identity from Clusal O multiple sequence alignment is reported.
In this study, neutral alignment is reported as 180°, with lower numbers indicating more varus malalignment.
Crucially, this means the 'best alignment' is reported for every read that can be mapped; if there are two alignments that are tied, then one is randomly chosen.
However, as previously indicated, only the transcript with the top scoring alignment is reported by MarkerMiner as a putatively orthologous single-copy transcript.
An algorithm for reference-guided assembly has three choices [ 13]: 1) to ignore (hence discard) all multi-reads; 2) to perform the best match approach, in which only the best alignment is reported or, if equally good best match alignments occur, one at random or all of them are reported; 3) to report all alignments up to a maximum number.
Similar(51)
In particular, an excellent alignment was reported in 67 and 77 %, of ESIN and percutaneous pinning groups, respectively.
The best alignment was reported for each candidate.
Only one best alignment was reported per read.
All alignments, no more than five points away from the best alignment are reported by Novoalign.
Unusually high densities of SNPs in some regions of the alignment were reported as further evidence of recombination.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com