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Alignment was additionally adjusted using Gblocks (File S3).
The alignments were additionally adjusted using Gblocks (supplementary file S1, Supplementary Material online).
All the individual alignments were additionally subjected to a likelihood mapping analysis using Tree-Puzzle [ 52, 53] (max. 10.000 quartets, WAG model [ 54] of substitution) in order to assess the phylogenetic signal in the dataset.
All significant NBPF alignment results were additionally investigated using the Pfam (Finn et al. 2010) protein database to determine homology to DUF1220 domains at the protein level.
In (f) a reconstruction including symmetrization, a medium Tikhonov-type filter and additionally 20 alignment iterations is shown, and finally further a reconstruction, that is additionally based on 5 images using (g) the HOFD derivative approximation or (h) the NR approach.
That is, additionally, absurd.
Additionally, since frameshifts are considered to be very rare events, their number in an alignment is restricted.
"The alignment is great".
Alignment is a tricky task.
Obviously, alignment is critical.
The alignment is deliberate.
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