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The alignment indicates a conserved backbone and the absence of cysteine residues, from where the block pattern L-x-H-x-L-x-Y-L-x-D-x(17,18 -A-x 17,18 -A-x 17,18 -A-xn be deduced (Fig. 4A).
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Phase alignment indicates an orderly temporal relationship between a stimulus and neural activity across successive stimulus epochs.
The alignment indicated a potential K+-binding domain conserved only in ASNase2 which leads to the suggestion that ASNase1 is a K+-independent ASNase, whereas ASNase2 could be a K+-dependent one.
Additionally, a multiple sequence alignment indicated a reliable similarity between T3SS needle-forming proteins and the Bcl-2 pore-forming region (Figure 2C).
The EST alignments were filtered based on requirements for minimum alignment length and sequence identity in order to differentiate cases in which the amino acid sequences were supported by a strong alignment from the cases in which the alignment indicated a paralog rather than a direct validation of the sequence by genomic data.
Genome-wide intraspecific sequence divergence was calculated from whole the genome alignment indicating a high level of sequence similarity.
The alignment indicated a spike gene nucleotide deletion at positions 164 169 (TTGGTG), which corresponded to amino acid deletion at positions 55 and 56.
The absence of nonsense mutations in the alignment indicated an absence of pseudogenes.
Where the alignment indicated an insertion, we inserted a segment of appropriate length with zero reads into the WS170 read profiles.
Sequence similarity between family members is low, with amino acid sequence alignment indicating an identity of approx. 10% between family members.
The alignments indicate a non-linear evolution of glyoxalase I enzymes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com