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Cell alignment increased with increasing surface roughness.
Alignment increased mRNAs and protein for early (MyoD) and late (MyoG, MyHC) myoblast markers in comparison to non-aligned matrices, and these levels corresponded with increased α7 protein.
RT-PCR, immunohistochemistry and immunofluorescence results showed that mimicking the tendon topography, i.e. increasing the substrate modulus as well as alignment increased the tenogenic differentiation.
Compared to randomly electrospun fibers, NFES fibers guided the extension of HEK 293T cells and the levels of cell alignment increased with decreasing fiber distances.
The hierarchical alignment activity, an instructional spatial analogy that employs structural, progressive, and hierarchical alignment, increased accuracy reasoning about temporal and spatial magnitudes in a lab-based setting (Resnick, Newcombe, et al., 2016).
The nanofibrous topography itself, independent of the fiber material, has demonstrated the potential to modulate cell behaviors desirable in tissue engineering such as: unidirectional alignment; increased viability, attachment, and ECM production; guided migration; and controlled differentiation.
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It has been shown that the removal of poorly aligned regions from an alignment increases the quality of subsequent analyses.
Scott et al. [23] reported that overcorrection of alignment increases the risk of degenerative change in the contralateral compartment.
Also, valgus alignment increases the risk of progression of lateral disease, and an increasing valgus angle is associated with more severe progression of lateral disease [2, 20, 21].
It was also found that surfactants cause the drops to align more in the flow direction and that, for both clean and surfactant-covered drops, this alignment increases with viscosity ratio.
Thus, as the number of sequences in a dataset surpasses their length, the memory required to double the number of sequences in an alignment increases at least four-fold and the time required increases at least eight-fold.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com