For reads longer than 1024bp, we would explore some specific alignment tools in future, such as BWA-SW [ 37].
Identified MAP pathways place current research into direct alignment in meeting the future challenge of creating a MAP-host interactome.
Predefined masking was selected to ensure reproducibility in future alignments, and finally, the alignment was refined with Gblocks (Castresana 2000) allowing 50% in coverage gaps.
We then propose knowledge-enabled, dynamic solutions that will hopefully pave the way to enhanced alignment construction and exploitation in future evolutionary systems biology studies.
By achieving this objective, we also produced a reference database of RAD markers that can be used for alignment of sequences generated in future work, and described in detail the properties of the coho linkage map.
Our specific objectives are first to construct a reference database of RAD sequences that can be used for alignments of sequences generated in future projects.
Since the axis of the lumen is in alignment with future embryonic axis, we have recently proposed that the on-time luminal closure is a critical factor that would guide and facilitate the correct embryonic orientation at initial implantation.
In future, such alignment should be contemplated in the planning phase of the priority setting effort rather that in the implementation phase.
In future, the alignment process may be automated, using motorized mirror mounts and optimizing for signal intensity of each channel separately.
Since knee alignment have great impact on biomechanical force transmission which may influence the degree of extrusion, a larger sample allowing enough numbers in different alignment categories would be valuable in future work [ 34].
