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Most strikingly, the alignment highlighted the universal conservation of the first leucine residue in the V regions of all animals.
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Even so, the current head of NSF's international programs believes that the new alignment highlights the need for every NSF program manager to be thinking about how to expand their portfolio internationally.
Additionally, a multiple sequence alignment highlights nucleotide polymorphisms within the consensus sequence.
A multiple sequence alignment highlighting the postions of disease-causing mutations is provided as additional information [Additional file 1].
The multiple sequence alignment highlights a sequence motif in the mid domain that binds the 5' phosphate of guide RNA.
The alignment highlights conserved hydrophobic residues (gray), other residues implicated in HAMP domain switching (red), and divergent substitutions (yellow).
(b) Alignment highlighting the use of the consensus (cic) vs. the alternative initiation codon (aic) in orchids and related outgroup species.
Manual refinement of these alignments highlighted five additional unannotated introns in genes HOP2, CGI121, YPR170W-B, YPR153W, and YPL109C, of which only the HOP2 intron is present in S. cerevisiae (the HOP2 intron is not yet in SGD, but was recently described by Chan et al. [2014]).
Whether in the form of acquisitions, partnerships or something else, these new alignments highlight an inescapable reality: Everything about how consumers discover, shop and buy is changing, and established retailers and brands must adapt.
The multiple alignments of the query sequence and protein sequences were also analysed using JOY [40], which produces formatted alignments highlighting unique patterns of amino acids substitutions in various environments, thus helping to identify misaligned regions or residues that play important structural role.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com