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To obtain a high specificity for the results, only the reads similar to a single transcript were computed, with a minimum similarity of 80%% and a read length alignment higher than 90%%.
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Prior to this value, the quality of the global alignment is higher than that of the local alignment, but above this value the global alignment quality decreases sharply, whereas the local alignment quality remains the same.
Among the 1,490 "unique bear EST clusters" without annotation, only nine contained more than five ESTs and seven had an alignment score higher than 50 when aligned with NT sequences.
Only the reads that were uniquely aligned to reference genome with read alignment score higher than 4.5 were retained for further analysis.
Furthermore, the conductivities parallel to the direction of m-CNT alignment were higher than those in the perpendicular direction, indicating that the alignment of the m-CNTs contributed to the enhancement of the anisotropic electrical properties of the composites in the direction of alignment.
The ESTs alignment lengths on the genome longer than 50% of ESTs lengths and the alignment identity higher than 85% were used as the cutoff for assigning significant homology.
Finally, we selected only those alignments with at least five reads supporting the same chimeric event, alignments defining (putative) introns with canonical splice sites (GT-AG) and an alignment quality higher than 40 (Phred scale).
That alignment was higher than the average for House Republicans.
Thus, in Sgo1-RNAi oocytes, the frequency of oocytes showing disordered chromosome alignment was higher than that in control (P<0.05) (Fig. 4C).
Alignment scores higher than the cut_tc threshold were considered significant.
Using alignment score higher than 100 as the cutoff, we annotated 778 "unique bear EST clusters" with known gene symbols.
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