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Finally (table 4), we compared the inferred percentages of PSGs between genes with high trace sequencing coverage, known annotation status, and 100% alignment HoT scores (henceforth "all good" genes) and genes that are deficient in all three criteria of coverage, annotation, and alignment (henceforth "all bad" genes).
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The alignment blocks (henceforth described as lncRNA exons) were further manually verified to annotate the exons.
Following assembly in PASA, 23,898 out of the 29,294 Trinity contigs (81%) had valid ME49 genome alignments and were clustered into 16,462 transcripts (henceforth PASA transcripts), while 5,396 had invalid alignments and were excluded from PASA analysis (Additional files 1, 2, 3 and 4).
Then we checked for misaligned sequences (henceforth called "outliers") in multiple amino acid sequence alignments (MSAs) of all OGs.
Figure S1 presents a sequence alignment of the feline KIT wild-type intron 1 with the LTR (henceforth the W allele), illustrating insertion breakpoints of the LTR element (GenBank id KC893343).
For every TSS, sequences 10 kbp upstream and 5 kbp downstream of the TSS (henceforth called the promoter sequence) and phastCons scores for multiple alignments of 30 vertebrate genomes were retrieved from UCSC.[6].
De-methyl-esterified HG (henceforth HGA) may affect assembly of the cellulose network, influencing the deposition and alignment of microfibrils in the wall [ 14].
Using these primer alignment criteria, putative autosomal target regions were identified for 245 of the 246 microsatellites ("ePCR fragments" henceforth).
To identify such sequence elements, we prepared separate alignments of sequences containing a C and those containing a T at the matK editing sites (henceforth called C-elements and T-elements, respectively).
42 clustalw was run either in default mode or with the gap opening and extension parameters optimized for RNA alignments (using the command-line switches-pwgapopen = 22.5 -gapopen = 22.5 -gapext = 0.83 -pwgapext = 0.83; henceforth referred to as clwopt).
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