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Contig assembly and multiple sequence alignments were performed using the "Assembly" and "Alignment" functions of Geneious, respectively.
Sequences were analyzed and compared using search and alignment functions of the IDNS™ Bacteria Module of SmartGene.
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Structures in each group were aligned by the multiple structure alignment function of RSmatch with default scoring matrices.
Protein gene sequences were aligned first for their amino acid sequences with the aid of ClustalX [ 69], with which nucleotide sequences were automatically aligned using the 'Import NBRF Protein Alignment' function of MacClade 4 (Sinauer Associates , Inc.
All the spot areas recognized by the automatic alignment function of the GenePix Pro were confirmed visually.
These alignments were then combined using the profile alignment function of ClustalX 1.83 [ 73].
Using the multiple alignment function of RSmatch, we generated a consensus structure for each structure group.
Deduced amino acid alignments were generated using the "create alignment" function of CLC-sequence viewer 6.9.1 based on the CLUSTALW alignment matrix/algorithm.
The pharmacophore alignment function of MOE was used to produce a rough initial alignment to ATP of the diverse input set based on common chemical features.
All these selected mouse structures were compared by the multiple structure alignment function of RSmatch which also gave the consensus structure.
Spots were matched using the alignment function of Progenesis SameSpots and Progenesis PG220 (version 2006; Nonlinear Dynamics, Durham, NC) matches were manually verified.
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