Exact(2)
Four subtype C reference sequences (accession numbers AY772699, U52953, U46016, and AF067155) were included in each subject's alignment for use as an out-group to root the trees.
Pairwise alignments of zfP2X4.1 and either hP2X4 or DdP2XA were prepared from the full alignment for use in the program Modeller [20].
Similar(58)
We thus hypothesized that current criteria are insufficient to build alignments for use with covariation analyses.
We show that current criteria are insufficient to build alignments for use with covariation analyses as systematic sequence alignment errors are present even in hand-curated structure-based alignment datasets like those from the Conserved Domain Database.
4) Trim alignments for use in phylogenetic analyses.
We located the corresponding genomic region in D. yakuba (droYak2.chr2L:7,042,800-7,053,500) using inter-species LASTZ alignments for use as an outgroup.
PAL2NAL [ 53] was used to make codon based nucleotide alignments for the use in MEGA and PAML.
Alignments were prepared for use in phylogenetic reconstructions as follows.
We created alignments for MAPP using three different methods.
The alignment used for the phylogenetic analysis was also used for this application.
Test data for alignment: We use the following three datasets to test the alignment accuracy of our method.
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