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The alignment efficiency of ortho-pyroxene was compared with those of various rock-forming minerals.
Very similarly for mRNA-seq, on average there were 168 million pair-end reads for each sample with alignment efficiency of 87% (Additional file 2 for sequence depth, alignment statistics, and number of genes detected with at least 1 tag and 1 RPKM for each individual sample from mRNA-seq).
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Given the different alignment efficiencies of software, clean reads were aligned using TopHat2 [ 74] (v2.0.12) with Bowtie [ 75] (v0.12.9.0) for single-end reads (49 bp) and Bowtie2 [ 76] (v2.1.0.0) for paired-end reads (90 and 150 bp).
Alignment efficiency (the ratio of uniquely aligned reads to total sequenced reads for each sample) ranged from 26.57% to 93.15% across all samples in this study (Table 1).
To avoid alignment efficiency biases caused by the presence of the insertion, the allele ratios were subsequently requantified using local realignment with the reference and mutated sequences.
We evaluated rRNA depletion efficiency and bias; library complexity; alignment efficiency; gene detection sensitivity and abundance; evenness of transcript coverage; and technical reproducibility.
At the same time, the composite in transverse direction to the alignment was totally insulating, indicating the efficiency of alignment.
Strained PDMS stamp can be applied to improve the efficiency of alignment yield and SiNW density.
There is uneven coverage across env which might be explained by sequence variation in the early part of the gene (see below), lowering the efficiency of alignment of reads to that region.
The efficiency of alignment-free measures also allows the reconstruction of phylogenies for whole genomes [ 4- 6].
The efficiency of the alignment propagation is higher for LCPs containing a short flexible spacer because of the stronger couplings between the chain backbone and the mesogenic groups.
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