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Alignment efficiency (the ratio of uniquely aligned reads to total sequenced reads for each sample) ranged from 26.57% to 93.15% across all samples in this study (Table 1).
In total, 3.67 gigabases (Gb) were sequenced, 2.74 Gb were aligned to the reference genome (average 74.66% alignment efficiency).
Aforementioned studies indicated that the FNN is helpful to improve the alignment efficiency.
The alignment efficiency of ortho-pyroxene was compared with those of various rock-forming minerals.
Depending on species, RNA-seq read alignment efficiency varied from 55 to 99% (Table S2).
This process was iterated over six cycles after which there was no significant gain in alignment efficiency.
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Given the different alignment efficiencies of software, clean reads were aligned using TopHat2 [ 74] (v2.0.12) with Bowtie [ 75] (v0.12.9.0) for single-end reads (49 bp) and Bowtie2 [ 76] (v2.1.0.0) for paired-end reads (90 and 150 bp).
Prior to each experiment, the confocal microscope was tested for field illumination alignment, optical efficiency, colocalization, and axial resolution (Lerner and Zucker 2004; Zucker 2006a, 2006b; Zucker and Lerner 2005; Zucker et al. 2007); and the lens was inspected and cleaned before use.
Results: Compared with other aligners, Slider has higher alignment accuracy and efficiency.
We evaluated multiple aligners based on criteria, including application-specific alignment feature, computational efficiency, and alignment accuracy.
We present the study on multiplexed beams automatic alignment of high efficiency excimer laser.
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