Exact(21)
In order to ensure the accuracy of sequence alignment, each pair of sequences were realigned with a pairwise aligner software LASTZ [ 36], then these InDels were extracted with an written Perl script.
With this proposal, the traditional experimental optical setups are considerably simplified, with the additional benefit that different masks can be utilized without needing to perform the setup alignment each time.
To direct the self-assembly of BCP thin film nanostructures, a suite of annealing techniques has been developed (e.g. thermal annealing, solvent vapor annealing, magnetic/electrical field alignment), each with its own set of controllable parameters and mechanisms for nanostructure reorganization.
After alignment, each image was segmented into discrete components by manually tracing every structure of interest (Fig. 10C).
For this alignment, each functional residue was evaluated against the expected functional residue from the CD consensus alignment.
HHpred searches against the Pfam database, and COACH comparison with the REP, Pfam and Established Repeats reference data were carried out as described in Methods (for COACH analysis against the Established Repeats data the matching 15 sequences were removed from the reference alignment each time).
Similar(39)
For each alignment of each segregant, read counts were tabulated for consecutive 5000-bp windows using cn.MOPS (Klambauer et al. 2012).
To improve the quality of local alignments, each upstream region was re-aligned using ClustalW [ 30].
Then the computer will move on to the next possible match in the database, and the next: millions of alignments each time.
Thus, we built 2183 alignments, each containing the orthologous sequences detected for a different E. coli protein.
Our two amino acid alignments and the two nucleotide (NT) alignments, each alignment having been generated by the two alignment methods described above, were then analyzed using ML, MP, and neighbor-joining (NJ) techniques (Figure 2).
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