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The number of SNPs in each of the subdomains was calculated from the structure-informed multiple sequence alignment described in the previous section.
We compared the three clustering algorithms (complete linkage, FastGroup and ESPRIT) by running them on the hand curated alignment described in the previous section.
All the eleven standard steps for carrying out alignment described in the section on ITools were used.
Subjects were recruited from the hospital of the surgeon authors as part of a prospective, randomized study of TKR alignment described in detail elsewhere [ 15].
Each alignment described in the previous section includes chains from the PDB that contain the relevant domain, EC assignment and bound ligand or catalytic site.
a Table values are percent differences in nucleotide sequence as determined from pairwise comparisons of the Mugsy alignment described in the Methods section.
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In this stage, the four sets of possible alignments described in Section 2 for each original read are computed by using the BWT.
The pairwise protein-protein alignments described in the previous section define the corresponding nucleotide positions between their respective mRNAs as well.
The quality of CORAL alignments between CDD-curated domains was evaluated against the reference alignments described in Section 2 and compared with alignments from COMPASS 3.0, HHalign 1.5.1.1 and PSI-BLAST.
A structure-based sequence alignment of proteins with favorable alignment scores (described in Section 2.4) is constructed in which the structurally aligned protein sequences are listed in the order of their decreasing alignment lengths.
We first used POPBAM to identify all variable sites in the alignment described above.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com