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Neck hypertonia is usually related to the abnormal static alignment derived from stooped posture.
The third method (conservation difference or CD) is based on the conservation differences between a native alignment and an alignment derived from the Z-score sequence design [ 21].
Then, every target structure was built with the MODELLER software (36), using local alignment derived from the above-described psi-blast search (37).
Struck & Fisse [ 13] found good support for Mollusca+Nemertea in Bayesian analyses of an amino acid alignment derived from EST data, while ML analyses were rather indifferent between Annelida and Mollusca as sister group to Nemertea.
A sequence alignment derived from the structural superposition of HsCENP-M with HsRab1A (PDB code 4FMC, [ Dong et al., 2012]) shows that CENP-M has lost essential sequence motifs required for GTP binding and hydrolysis by small G-proteins (Vetter and Wittinghofer, 2001; Figure 3C).
The topology of the maximum likelihood tree based on a direct nucleotide alignment (edited with ALISCORE; including 12,648 positions; Additional file 5) does not differ from that based on the nucleotide alignment derived from the amino acid alignment in any strongly supported nodes.
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We explored further the robustness of the phylogenetic signal of the datasets against a) the alignment deriving after highly variable regions were removed with even more stringent criteria by Gblocks and b) the alignment deriving from the default settings in ClustalX.
We produced nucleotide alignments derived from amino acid alignments of the 20 V1R sequences ("codon-aligned"; see Additional file 1), as well as homologous sequences from musculus and rat.
Both of the above two algorithms use optimal alignments derived from LVCSR results, which may implicitly incorporate pronunciation and grammar constraints.
We use alignments derived from an all-on-all sequence comparison to define domains within protein sequences based on a global maximum likelihood model.
A triphone system was then constructed based on the alignments derived from the monophone system, and a linear discriminant analysis (LDA) transform was employed to select the most discriminative dimensions from a large context (five frames to the left and right, respectively).
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