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a Microsatellites whose repeat unit is not composed exclusively of the nucleotides A and T, b Total numberof microsatellites occurring in a region where the alignment depth is greater than one, c Total numberof microsatellites for which we observed more than one allele in our EST collection.
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A cannula was inserted over the dilator, and proper alignment and depth were ensured before dilator removal.
The maximum alignment depth observed was 344 ESTs in one contig.
The nucleotide diversity indicator π (average proportion of nucleotide differences between all possible pairs of sequences) [ 45] was calculated for each alignment using the effective length of the multiple sequence alignment (not considering regions of the alignment where the depth was 1) as the total number of available sites.
This alignment-based algorithm still provides predictions even when the coverage depth is as low as three fold [ 21].
On microgrooved surfaces, groove depth is one of the most important parameters in defining cell alignment.
Depth is building rapidly.
But depth is an issue.
Standard depth is around 12-14".
The resulting alignment depths, which are greater than those typically seen in 5' projects, together with our deliberate use of outbred individuals for building the cDNA libraries (Table 1), enabled us to identify SNPs in many of our UniGenes.
Coverage depth was nonuniform alignment metrics were suboptimal for FFPE data, as described in multiple studies.15,19,22,33 However, our results were influenced by lower stringency of fragment size selection and the polymerase chain reaction step included in the FFPE library preparation method (see Materials and Methods) to improve library yield.
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