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ZP subfamilies (one sequence alignment dataset for ZPAX and ZPB, and another for ZPC) were aligned with mafft 6 (http://mafft.cbrc.jp/alignment/server/) and a ZPA homolog from Xenopus levis was used as an outgroup.
The alignment dataset was analyzed using maximum likelihood (ML), maximum parsimony (MP) and Bayesian methods.
Table 2 Experimental results summary Pairwise alignment Multi-view alignment Dataset Number of RI pairs Avg.
A concatenated alignment dataset was produced by putting each ortholog alignment in series using Bioedit v7.2.0 (Hall, 1999).
Open image in new window Figure 5 Phylogenetic network computed from the concatenated orthologs alignment dataset using the neighbornet algorithm and the uncorrected distances.
After the 127 ortholog concatenated alignment dataset was produced, and its model was selected as RtREV + I + G + F (gamma shape = 0.947; proportion of invariable sites = 0.063), a concatenated phylogenomic tree was calculated using both maximum likelihood and Bayesian inference methods (Fig. 4).
Similar(29)
We achieve state-of-the-art performance in the most well-known image-text alignment datasets, namely Microsoft COCO, Flickr8k, and Flickr30k, with a method that is conceptually much simpler and that possesses considerably fewer parameters than current approaches.
Consequently, two alignment datasets were generated: 1. 817 sites of 30 sequences (16S) and 2. 461 sites of 26 sequences (COI).
The cd00300 alignment showed that misalignments occur in structure-based alignment datasets; importantly, cd00300 showed that misalignments occur in functionally-important conserved regions.
We show that current criteria are insufficient to build alignments for use with covariation analyses as systematic sequence alignment errors are present even in hand-curated structure-based alignment datasets like those from the Conserved Domain Database.
Then, each gene portions were concatenated to make the above alignment datasets.
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