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Sequence alignment data showed that some snoRNAs sequences diverged even among closely related primates.
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Structural alignment data shows that the analysis those paralogous genes based on indels yields results that would be compatible with the rooting proposed by Cavalier-Smith.
Despite the prediction of an N-terminal EP for EutG (EUT-associated alcohol dehydrogenase) from Escherichia coli based on a sequence alignment, experimental data showed that the first 19 amino acids of EutG from Salmonella enterica do not target fused GFP to the shells.
A nucleotide alignment (data not shown) shows that the three BKJ coding regions only differ by one or two nucleotide changes and therefore have a greater than 99% identity between the three sequences.
The alignment data clearly showed overall sequence homology among the three species from different mammalian subgroups.
Although the long-branch taxa were removed, the ML trees inferred from the four different reduced alignment data sets showed that the pycnogonids are consistently clustered with the terrestrial spider H. langzhouensis [See additional file 5A, C, D], except for the ML tree with the 21 taxa in which A. bituberculata appears as a sister group of euchelicerates [See Additional file 5B].
The same gold standard dataset was used by Major et al. (2013), who also examined the reliability of calling HLA alleles using NGS, but using the 1000 Genomes alignment data, and showed that this publicly available dataset can be used for this purpose, after appropriate filters (e.g., coverage) are applied.
Examination of 40 random near-identical paralogous alignments (data not shown) shows that the majority (>60%) of the differences are indels in the vicinity of homopolymer runs which very likely derive from the large proportion of 454 STRs used in the TA construction.
Visual inspection of image data showed effective alignment of the computed trajectories with the basal turn.
About 38% of them showed at least one base mismatch over the 16-25 bp alignment (data not shown).
Sequences shorter than 100 amino acids were omitted from analyses as those tended to align poorly even in a guided alignment (data not shown).
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