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The entire related 16S rRNA gene sequences were downloaded from the database (http://www.ncbi.nlm.nih.gov/) aligned using the CLUSTAL_X program [ 9] and the alignment corrected manually.
Positions with a quality score of < 40 were cross-referenced to the multiple genome alignment, corrected where possible, or changed to an "N".
Briefly, CAPS compares the correlated variance of the evolutionary rates at 2 amino acid sites in a protein alignment, corrected by the time since the divergence of the 2 sequences they belong to.
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Multi-sequence alignments were made using MAFFT software (http://align.bmr.kyushu-u.ac.jp/mafft/software/) with the "G-INS-I" setting for global alignments, corrected manually.
The alignment was corrected manually using BioEdit sequence alignment editor (Hall, 1999).
We adopt the concept of a correct alignment from Langmead and Salzberg [ 8], who determined an alignment correct only if the alignment was on the same strand and the leftmost position was within 50 bp of the assigned position.
This procedure, addresses measurement variations from gel to gel while pixel level alignment corrects for positional variation from gel to gel.
The first step performs a pre-detection of signatures alignment, and in the second step the alignment is corrected and the signatures are detected by a matcher.
All alignments were corrected for obvious alignment ambiguity.
Sequences were edited, aligned on S EQUENCHER 3.0 (Gene Codes Corporation) and the alignment manually corrected using S EAVIEW[ 48].
The sequences were aligned by using the phylogeny tree program [ 15], and the alignment was corrected manually.
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