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Protein subsequences translated from aligned contig subsequences and the alignment coordinates were extracted from tabular blast output; corresponding DNA subsequences were extracted from contigs.
This alignment looks for inversions by attempting to align the query gap sequence to the genomic region bounded by the genomic alignment coordinates of the bases flanking the gap in the original contig-to-genome alignment.
In order to compare these alignments, all DANGER sequences were mapped onto the Mab-21 profile sequence according to the pairwise alignment coordinates.
To maintain consistency between mouse genome assembly and our alignment coordinates, the multi-species alignment was compressed such that the mouse sequence is ungapped.
Exon templates were then inferred from EST alignment coordinates.
PCR duplicates were identified based on read start and end alignment coordinates.
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Spine then outputs DNA sequence and genomic coordinates of regions present in a user-defined subset of the reference genomes using the NUCmer alignment coordinate file and the sequences of the reference genomes.
For assembled genomes without S288c alignments, coordinates were obtained by blast.
Predictions are clustered if they share the start, middle (m i d d l e = s t a r t + e n d 2 ) or end (alignment- coordinate, their length in balignment- coordinate and atheirst one of the folengthg condinions is true: The names of the transcription factor baseing sites pairscted are sisilar (notheonsamering upper/lower cand, spates, numbers).
For each comparison, a report containing sequence identifiers, alignment length, coordinates of the most similar regions, percentage of identity, number of gaps, raw and bit scores and E-value was returned.
FF participated in the sequences alignment and coordinated the co-authors.
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