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This alignment contained a much smaller number of sites (results not shown), however the results are similar to the results obtained for the four species alignment (Fig. S6).
This 763-locus alignment contained a total of 28,324 parsimony informative characters.
Each line of the multiple alignment contained a distinct set of contigs spanning the maximum possible number of nucleotide positions of the reference sequence.
Some bacterial taxa were not represented in the LTP database; therefore, the alignment contained a subset of the taxa represented in each original MIC distribution [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1104650)].
The top scoring BLASTp alignment for every query protein was considered a "match" if the identity was greater than 75% of the full length and if the alignment contained a perfect match string of at least 20 amino acids [ 29].
If an amino acid position in the concatenated alignment contained a gap at a position in any of the species, or in multiple species, that position was removed prior to analysis by using Gblocks 0.91b (Talavera and Castresana 2007), as we did not use a model of sequence evolution that allowed for insertions or deletions.
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The complete alignment contains a total of 3,290,621 aligned positions (2,104,923 and 1,185,698 per chromosome), with 0.10% of nucleotide changes in aligned regions (2,195 and 1,200 nucleotide changes, respectively).
The alignment contains a number of gaps higher than 30% of the aligned bases.
For example, consider below an alignment containing a hinge rotation about residue 10 of T (marked by *).
The optimal alignment contains a cluster of 4 ORFs whose products all interact with each other in both viruses.
Taxa that contain a gap in this column under the singlet alignment contain A residues according to the triplet MAP alignment, decreasing the support for (10,12,18) clade.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com