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The MT, HEL and RdRp domains identified by Pfam as described above were extracted from the library sequences, aligned by ClustalX, and combined into a new alignment consisting of only these domain regions.
A set of single copy gene families present in all genomes were then selected and aligned using MUSCLE [46], and concatenated into a single alignment consisting of a total of 196,481 amino acid positions (476 genes/ORFs, see Table S4).
128 of these sequences were used in an alignment consisting of ∼100 000 aa for Bayesian analysis.
A block is then defined as a region in the alignment consisting of at least 3 columns, in which the NorMD is above the threshold value of 0.2.
We assembled an alignment consisting of concatenated amino acid sequences from the fHANT-AC for all available fungal genomes (as of May, 2006, shown with an * in SI Table S1) and also Phytophthora sojae and Phytophthora ramorum genomes, which were included as the outgroup.
An alignment consisting of 229 amino acid positions was obtained.
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Up to this stage, the alignment consists of aligned representatives of NCIV clusters.
The RPE alignment consisted of 183 alignable characters and the GAPDH alignment comprised 278 alignable characters.
The longest Galinter alignment consists of 117 aligned NCIVs, and the results are visualized in Figure 3A and 3B.
This nucleotide alignment consisted of 1,915 unambiguously aligned sites, once gaps and ambiguously aligned regions were removed.
The resulting alignment consisted of 618 columns of aligned amino acids.
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