Your English writing platform
Discover LudwigExact(17)
In the original NAST implementation, each user-submitted candidate sequence is aligned to the Greengenes 'Core Set' (a template alignment), comprising approximately 5000 aligned non-chimeric sequences representative of the currently recognized diversity among bacteria and archaea.
Instead, a codon alignment comprising 86.5% of the total CDS and without stop codons (Figure S1) was used.
The ITS rDNA alignment comprising 165 sequences had a total length of 983 positions, 221 of which were excluded because they mainly contained leading or trailing gaps.
For each gene region, a multiple alignment, comprising DNA sequences of unequal length for 26 terminal taxa, was generated using default parameters in Clustal X v.1.83.1 [23].
An alignment comprising 203 taxa and 493 characters was generated and analyzed using parsimony, resulting in one most parsimonious tree (Figure 1).
For a second alignment, comprising bat orthologues, the MAPP score of each amino acid variant was derived, together with the score of all other variants at the same site in the alignment that differed from the ancestral sequence by an equal or fewer number of nucleotide substitutions.
Similar(43)
The RPE alignment consisted of 183 alignable characters and the GAPDH alignment comprised 278 alignable characters.
The resulting 123 taxa prank alignment comprises 9,027 aligned positions and 2,051 positions past trimming.
After removing unreliably aligned regions, the final alignment comprised 150 taxa and 1,647 positions (the alignment is available upon request).
After indels and poorly aligned positions were removed the alignment comprised 15,685 bp including 6,986 informative sites.
The resulting alignment comprised 102 WNTs and 220 positions (available upon request).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com