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This resulted in a multiple sequence alignment centered on the query sequence.
We eliminated from consideration any sequences containing gaps in the 9-aa region of the alignment centered on the US-encoded peptide.
The most highly conserved region in the whole alignment is centered on a Cys residue in the motif Cys-Xaa-Gly (bold type indicates residues found in the catalytic triad of sortases).
In target shooting there are two approaches to sight alignment: Center of Mass (aim at the center of the bullseye) or 6 o'clock hold (aim just underneath of the bullseye).
The greatest local sequence conservation in a sortase multiple sequence alignment is centered on the active site Cys.
Format the cells alignment center.
Many of the great spiritual masters teach that you should never take action, make a move or decision, until you feel in complete alignment, clear and centered.
Select the shapes which are to lie in a vertical straight line with one another and center them with Modify > Alignment > Centered.
Tilted particles were centered by translational alignment to mass centered class averages using IMAGIC [23].
Quickly draw it back in and return to center alignment Twist to your right, shoot the ball out, draw it in and return to center.
For those on a horizontal line select the shapes and center them with Modify > Alignment > Center.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com